# April 8, 2020 - Continuing New Image Segmentation Method

### Lab Work

Today, I continued working on the new image segmentation method by following the instructions from this article. Last time, I had been able to produce a color threshold graph for eggs, but I realized that the graph that was produced was much more rectangular than the graphs that seemed to be produced in the article. Then, I realized that it was because I did not add in the LogNorm() function, but once I had done so, I produced this graph:

I was also able to produce a combined image for the sperm screenshots as well as produce a color space graph:

combined image:

graph:

In terms of comparing the two graphs that were produced, it seems like both egg and sperm coloration is very similar when seen in RGB color spaces (the G and B values for both gonads overlap quite a bit), so the RGB color space may not be the best option in terms of deciding what color space to use to threshold the images.

### Next Steps

Create graphs from different color space options to see if there is a better way to segment the images